Thermo Fisher Scientific PRDX3 Monoclonal Antibody (6E6E12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Flow Cytometry (Flow)

1:50-1:200

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6E6E12

Immunogen

Recombinant Human Thioredoxin-dependent peroxide reductase, mitochondrial protein (63-256AA) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PRDX3,uniProtId:P30048-1,ncbiNodeId:9606,antigenRange:63-256,antigenLength:256,antigenImageFileName:MA5-50291_PRDX3_P30048-1_House_mouse.svg,antigenImageFileNamePDP:MA5-50291_PRDX3_P30048-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.85 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

RRID

AB_3091045

Target Information

Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20-30 kilodalton in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.