Thermo Fisher Scientific KLF4 Monoclonal Antibody (1E5)
Human KLF4 단백질을 검출하는 Mouse monoclonal antibody (Clone 1E5). Western blot, IHC, ELISA 등 다양한 응용에 사용 가능. Recombinant KLF4(AA 2–470) 항원을 이용해 제작. 55kDa의 KLF4 단백질 검출에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | – | View 2 publications |
| ELISA | 1:10,000 | – |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1E5 |
| Immunogen | Purified recombinant fragment of human KLF4 (Amino Acid: 2–470) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4 °C short term; for long term, store at −20 °C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10984501 |
Product Specific Information
MA5-15672 targets KLF4 in indirect ELISA, IHC, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human KLF4 (Amino Acid: 2–470) expressed in E. coli.
It detects KLF4 with a predicted molecular weight of approximately 55 kDa.
Target Information
Kruppel-like factor 4 (KLF4) is encoded by the KLF4 gene located on chromosome 9 in humans.
Also known as gut-enriched Kruppel-like factor (GKLF) or epithelial zinc-finger protein (EZF), it belongs to the Kruppel-like factor family of DNA-binding zinc finger transcription factors.
KLF4 plays roles in endothelial signaling, adipogenesis, differentiation, embryogenesis, and cell cycle control.
As one of the four Yamanaka factors, it is essential for reprogramming differentiated cells into pluripotent stem cells.
Its localization influences function — nuclear localization is associated with oncogenic activity, while cytosolic localization is linked to tumor-suppressive effects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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