Thermo Fisher Scientific IkB beta Monoclonal Antibody (7B4)
Human NFKBIB 단백질을 인식하는 Mouse IgG1 단일클론 항체. Western blot, ICC/IF, ELISA에 적합. 비결합형 액상 형태로 제공되며, 단기 4°C 보관 및 장기 -20°C 보관 권장. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7B4 |
| Immunogen | Purified recombinant fragment of human NFKBIB expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10982569 |
Product Specific Information
MA5-15673 targets NFKBIB in indirect ELISA and WB applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of human NFKBIB expressed in E. coli.
MA5-15673 detects NFKBIB, which has a predicted molecular weight of approximately 45 kDa.
Target Information
The NF-kB/Rel transcription factors are present in the cytosol in an inactive state, complexed with inhibitory IkB proteins.
Activation of NF-kB typically occurs through phosphorylation-induced, proteasome-mediated degradation of IkB.
The key regulatory step involves activation of the IkB kinase (IKK) complex, composed of three subunits: IKKα, IKKβ (catalytic), and IKKγ (regulatory).
Phosphorylation at Ser177 and Ser181 of IKKβ (Ser176 and Ser180 in IKKα) induces conformational changes leading to kinase activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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