Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Biotin, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Biotin, eBioscience™

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV Series (395–805)
• Brilliant Violet Series (421–786)
• eFluor 506
• FITC
• NovaFluor Blue, Red, Violet, Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright Series (436–780)
• Custom conjugation available upon request

Product Specific Information

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associates with integrin beta2 (CD18) and is expressed on monocytes, granulocytes, activated lymphocytes, and a subset of NK cells. It serves as a receptor for CD54, CD102, CD50, and iC3b, mediating cell-cell and cell-matrix interactions.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Validated for flow cytometric analysis of normal human peripheral blood cells
• Recommended Usage: ≤ 1 µg per test (100 µL final volume, 10⁵–10⁸ cells/test)
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD11b (integrin alpha-M, ITGAM) forms a heterodimer with CD18 (integrin beta-2), known as Mac-1 or CR3. It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia. CD11b/CD18 functions as a receptor for ICAMs, fibrinogen, Factor X, and iC3b, playing a crucial role in adhesion and immune response.

CD11b expression varies across tissues:

• ~8% of spleen cells
• ~44% of bone marrow cells
• <1% of thymocytes

Commonly used as a microglial marker in nervous tissue. Expression increases during monocyte maturation. Dysregulation is associated with autoimmune diseases such as systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.