Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Biotin, eBioscience
마우스 CD11c 인테그린 알파X를 인식하는 N418 단클론 항체로, Biotin으로 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 고순도 친화 크로마토그래피로 정제되었습니다. 4°C에서 보관하며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 20 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 9 publications |
| Immunocytochemistry (ICC/IF) | - | 8 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | 146 publications |
| Functional Assay | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 11 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N418 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466362 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Brilliant UV 395–805 series
- Brilliant Violet 421–711 series
- eFluor 450, 506, 615, 660
- FITC
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 780
- NovaFluor Blue 510–690, NovaFluor Yellow 570
- Functional Grade
- Custom conjugation available upon request
Product Specific Information
The N418 monoclonal antibody reacts with mouse CD11c (integrin alphaX). CD11c associates with beta2 integrin to form the CD11c/CD18 heterodimer. It is expressed by dendritic cells, subsets of intestinal intraepithelial lymphocytes, and some activated T cells. CD11c/CD18 interacts with CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.
N418 binds to CD11c on splenic dendritic cells in mouse spleen and precipitates a 150/90 kDa heterodimer.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg/test in 100 µL final volume. Optimal titration required.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD11, together with CD18, forms a heterodimer adhesion molecule.
- CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
- CD11b: Found in monocytes, macrophages, NK cells, some B/T cells, granulocytes
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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