Thermo Fisher Scientific Cytokeratin 5/8 Monoclonal Antibody (SPM268)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

1-2 µg/mL

-

Flow Cytometry (Flow)

1-2 µg/10^6 cells

-

Product Specifications

Species Reactivity

Bovine, Dog, Ferret, Hamster, Human, Mouse, Sheep, Pig, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

SPM268

Immunogen

Cytoskeletal preparation from HeLa cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 5/8,uniProtId:P05787-1,ncbiNodeId:9606,antigenRange:1-483,antigenLength:483,antigenImageFileName:MSM13X-948-P1ABX_Cytokeratin_58_P05787-1_House_mouse.svg,antigenImageFileNamePDP:MSM13X-948-P1ABX_Cytokeratin_58_P05787-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:Cytokeratin 5/8,uniProtId:P13647-1,ncbiNodeId:9606,antigenRange:1-590,antigenLength:590,antigenImageFileName:MSM13X-948-P1ABX_Cytokeratin_58_P13647-1_House_mouse.svg,antigenImageFileNamePDP:MSM13X-948-P1ABX_Cytokeratin_58_P13647-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Does not react with Chicken or Xenopus

Target Information

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.