Thermo Fisher Scientific CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), APC, eBioscience

Thermo Fisher Scientific CD11a (LFA-1α) Monoclonal Antibody (M17/4), APC, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 5 publications

Product Specifications

Additional Formats

• Unconjugated
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The M17/4 monoclonal antibody reacts with mouse CD11a, a 180 kDa integrin αL (LFA-1α chain). LFA-1, formed by non-covalent association of CD11a with CD18 (integrin β2), functions as an adhesion molecule involved in lymphocyte and granulocyte activity. Ligands include CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3). CD11a is expressed by all leukocytes.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD11a (LFA-1α, ITGAL) pairs with CD18 to form LFA-1 (αLβ2 integrin). It mediates leukocyte adhesion via ICAM-1, -2, and -3 and contributes to lymphocyte co-stimulatory signaling. CD11a exists in low- and high-affinity conformations depending on cellular activation. It plays roles in immune cell interaction, tissue localization, differentiation, proliferation, and T-cell effector functions. Blocking CD11a is a therapeutic strategy for inflammatory diseases; for example, Efalizumab (Raptiva) targets CD11a to modulate T-cell migration. Two transcript variants exist. Associated diseases include benign cephalic histiocytosis and leukocyte adhesion deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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