Thermo Fisher Scientific CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), APC, eBioscience
인간 Mac-1 활성 특이 에피토프를 인식하는 CBRM1/5 단클론 항체로, APC 형광으로 표지되어 있습니다. Flow cytometry 분석에 최적화되어 있으며, 5 µL/test로 사용합니다. 활성화된 단핵구 및 호중구에서 Mac-1 결합을 차단합니다.
- 카탈로그번호
- 17-0113-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), APC, eBioscience
Thermo Fisher Scientific CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), APC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 14 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CBRM1/5 |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10596976 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. It binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters, but not on resting myeloid cells. Mac-1 participates in adhesive cell interactions, and this antibody recognizes an epitope localized to the I domain on the alpha chain near the ligand binding site.
CBRM1/5 blocks Mac-1-dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to ICAM-1.
Low-level activation may occur during processing of freshly drawn blood, showing some binding in unstimulated samples. Higher Mac-1 expression is observed in activated samples compared to resting cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometry on stimulated peripheral blood cells.
Use at 5 µL (0.5 µg) per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD11b (integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule that associates with integrin beta-2 (CD18). The CD11b/CD18 complex (Mac-1, CR3) is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cell subsets, dendritic cells, and microglia.
It functions as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X — mediating critical cell-cell and cell-matrix interactions.
CD11b expression varies across tissues: ~8% of spleen cells, 44% of bone marrow cells, <1% of thymocytes. Commonly used as a microglial marker.
Expression increases during monocyte maturation; peritoneal macrophages express higher levels than splenic macrophages.
Dysfunction of CD11b is associated with systemic lupus erythematosus and ITGAM-related susceptibility to autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.