Thermo Fisher Scientific CD15 Monoclonal Antibody (MMA), APC, eBioscience
인간 CD15(Lewis X) 항원을 인식하는 MMA 단클론 항체로, APC 형광체가 결합된 Flow Cytometry용 시약입니다. 인간 백혈구 분석에 적합하며, 633–647 nm 적색 레이저에서 660 nm 방출을 나타냅니다. 4°C에서 암소 보관하며 동결 금지.
- 카탈로그번호
- 17-0158-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD15 Monoclonal Antibody (MMA), APC, eBioscience
Thermo Fisher Scientific CD15 Monoclonal Antibody (MMA), APC, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 9 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgM |
| Recommended Isotype Control | Mouse IgM Isotype Control (11E10), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MMA |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573138 |
Additional Formats
- Alexa Fluor 514
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Alexa Fluor Plus 750
- eFluor 450
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The MMA monoclonal antibody reacts with human CD15 (Lewis X), a 3-fucosyl-N-acetyllactosamine carbohydrate moiety expressed by granulocytes. Monocytes express this structure at varying degrees, while lymphocytes are negative.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells.
Recommended usage: 5 µL (0.125 µg) per test (final volume 100 µL). Cell number may range from 10⁵–10⁸ cells/test.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of various cell types including granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system, and tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X. High Le(x) expression promotes calcium-dependent self-aggregation, involved in embryo compaction and teratocarcinoma cell autoaggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands. CD15 expression is used to confirm Hodgkin’s disease diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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