Thermo Fisher Scientific CD15 Monoclonal Antibody (MMA), APC, eBioscience

Thermo Fisher Scientific CD15 Monoclonal Antibody (MMA), APC, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 9 publications

Product Specifications

Additional Formats

• Alexa Fluor 514
• Alexa Fluor Plus 594
• Alexa Fluor Plus 647
• Alexa Fluor Plus 750
• eFluor 450
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The MMA monoclonal antibody reacts with human CD15 (Lewis X), a 3-fucosyl-N-acetyllactosamine carbohydrate moiety expressed by granulocytes. Monocytes express this structure at varying degrees, while lymphocytes are negative.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells.
Recommended usage: 5 µL (0.125 µg) per test (final volume 100 µL). Cell number may range from 10⁵–10⁸ cells/test.

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of various cell types including granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system, and tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X. High Le(x) expression promotes calcium-dependent self-aggregation, involved in embryo compaction and teratocarcinoma cell autoaggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands. CD15 expression is used to confirm Hodgkin’s disease diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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