Thermo Fisher Scientific CD85k (Gp49b) Monoclonal Antibody (H1.1), Functional Grade, eBioscience
마우스 gp49A/B 인식 단클론 항체로, NK 및 골수세포의 기능적 분석에 적합. Flow cytometry와 functional assay에 사용 가능. 기능 등급(Functional Grade)으로 세포 활성 억제 연구에 활용. 고순도, 저엔도톡신 항체.
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카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD85k (Gp49b) Monoclonal Antibody (H1.1), Functional Grade, eBioscience
Applications
Flow Cytometry (Flow)
- Tested Dilution: ≤ 1 µg/test
- Publications: View 1 publication
Neutralization (Neu)
- Assay-Dependent
Functional Assay (Functional)
- Assay-Dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H1.1 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2866040 |
| Filtration | 0.2 µm post-manufacturing filtered |
| Purity | >90% (SDS-PAGE) |
| Endotoxin Level | <0.001 ng/µg antibody (LAL assay) |
| Aggregation | <10% (HPLC) |
Product Specific Information
The monoclonal antibody H1.1 recognizes gp49, a receptor structurally similar to PIR and ILT/KIR receptor families.
Two closely linked genes on mouse chromosome 10 encode gp49A and gp49B, which share ~90% identity in extracellular regions but differ in cytoplasmic domains. gp49B contains an ITAM domain, while gp49A is short and lacks it.
gp49 expression was first identified on mast and LAK (IL-2 activated NK) cells, with LAK cells expressing predominantly gp49B. gp49 is also found on myeloid cells. Although its detailed function is not fully known, gp49B can inhibit cellular activation.
The H1.1 antibody recognizes both gp49A and gp49B. When crosslinked with anti-NK1.1, it can prevent cytokine release in KY-2 cells.
It has been reported for flow cytometric analysis and functional assays, and shown to block cytokine release.
Recommended usage:
- Tested in flow cytometry using mouse bone marrow cells
- ≤ 1 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
- Titrate for optimal performance
Target Information
This gene belongs to the leukocyte immunoglobulin-like receptor (LIR) family located at chromosomal region 19q13.4.
The encoded protein is a subfamily B LIR receptor, containing two or four extracellular Ig domains, a transmembrane domain, and ITIM motifs.
It is expressed on immune cells, binds MHC class I molecules, and transduces inhibitory signals that regulate immune activation.
It may also function in antigen capture and presentation, helping to control inflammation and prevent autoreactivity.
Multiple transcript variants encoding different isoforms have been identified (RefSeq, Jul 2008).
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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