Thermo Fisher Scientific Ly-6C Monoclonal Antibody (ER-MP20)
Rat monoclonal antibody (clone ER-MP20) specific for murine Ly-6C antigen. Suitable for IHC, IHC (Frozen), Flow Cytometry, and Immunoprecipitation. Recognizes macrophage and dendritic cell precursors. Supplied unconjugated in PBS with sodium azide. For...
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Flow Cytometry (Flow) | 1:10–1:50 | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ER-MP20 |
| Immunogen | Balb/c macrophage precursor cell hybrids |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_934514 |
Additional Formats
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Rat anti-Mouse Ly-6C antibody, clone ER-MP20, recognizes murine Ly-6C, a 131 amino acid (~14 kDa) differentiation antigen expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts, and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.
Target Information
Granulocytes are a category of white blood cells characterized by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) due to the varying shapes of the nucleus, typically lobed into three segments. The term "polymorphonuclear leukocyte" often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by regulatory complement proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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