Thermo Fisher Scientific PDHB Recombinant Rabbit Monoclonal Antibody (JM66-33)
PDHB 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, Flow Cytometry에 적합합니다. 높은 특이성과 재현성을 제공하며, 로트 간 일관성이 우수합니다. 단백질 A로 정제된 액상 항체로 연구용에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM66-33 |
| Immunogen | Synthetic peptide within Human Pyruvate Dehydrogenase E1 beta subunit (aa 310–359) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810034 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
PDHB (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial), also known as PHE1B, is a mitochondrial enzyme that forms part of the pyruvate dehydrogenase multienzyme complex (PDH).
It catalyzes the first reaction in the oxidative decarboxylation of pyruvate to acetyl-CoA and CO₂.
Defects in PDHB cause pyruvate dehydrogenase E1-beta deficiency (PDHBD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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