Thermo Fisher Scientific Angiopoietin 2 Recombinant Rabbit Monoclonal Antibody (JM71-34)
Angiopoietin 2 단백질을 표적하는 재조합 토끼 단클론 항체로, 높은 특이성과 재현성을 제공합니다. Western blot, IHC, Flow cytometry 등 다양한 응용에 적합하며, HEK293 세포 발현 시스템으로 생산된 동물 유래 성분 없는 항체입니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | 1 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM71-34 |
| Immunogen | Synthetic peptide within Human Angiopoietin 2 (aa 351–400) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium Azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810036 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences are cloned from immunoreactive rabbits, and individual clones are screened for optimal production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
Angiopoietin 2 (ANG-2) binds to the endothelial cell receptor Tie-2 but does not induce tyrosine phosphorylation. ANG-2 modulates ANG-1 activation of Tie-2 and can act as either an agonist or antagonist depending on context.
ANG-1, ANG-2, Tie-2, along with ANG-3 and ANG-4, are essential for embryonic and adult angiogenesis.
ANG-2 is a secreted protein (480 amino acids) composed of:
- N-terminal coiled coil domain (alpha-helix-rich)
- C-terminal fibrinogen-like domain
ANG-2 predominantly exists as a disulfide-linked dimer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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