Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 660, 700, Plus 594
• APC, APC-eFluor 780
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Custom conjugation available upon request

Product Specific Information

The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, and mitosis) but absent in resting cells (G0). Ki-67 redistributes from the nucleus to chromosomes during mitosis and serves as a marker for cellular proliferation. The Ki-67 labeling index is used to predict outcomes in certain cancers. Ki-67 interacts with chromobox protein homolog 3 (CBX3).

The SolA15 antibody also recognizes human, non-human primate, and canine Ki-67.

Applications Reported:

• Intracellular staining and flow cytometric analysis
• Immunohistochemistry (frozen and paraffin-embedded tissue)
• Microscopy and immunocytochemistry

Applications Tested:

• Immunocytochemistry of fixed and permeabilized C2C12 cells (≤ 5 µg/mL)
• Intracellular staining and flow cytometry of stimulated mouse splenocytes (≤ 0.125 µg/test) using Foxp3/Transcription Factor Buffer Set (Product #00-5523-00)
• Refer to BestProtocols® for intracellular staining of nuclear proteins (Protocol B)
• Antibody should be titrated for optimal assay performance
• 0.2 µm post-manufacturing filtered

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin through its FHA domain and shows cell cycle–dependent localization: perinucleolar at G1, nuclear matrix at G2, and chromosomal during M phase. Ki-67 is widely used as a proliferation marker since it is absent in G0 cells. It is useful in determining the growth fraction of neoplasms and correlates with tumor grade and prognosis. Ki-67 is also used as a neuronal marker of cell proliferation.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.