Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Biotin, eBioscience
Ki-67 단백질을 인식하는 SolA15 단클론 항체로, 세포 증식 마커 연구에 사용. 다양한 종에 반응하며 Flow Cytometry, IHC, ICC 등 다중 응용 가능. Biotin 결합형으로 고감도 검출 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 |
| Immunohistochemistry (IHC) | - | 66 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | 3 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 1 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | 41 |
| Flow Cytometry (Flow) | 0.125 µg/test | 51 |
| Functional Assay | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SolA15 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572793 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 660, 700, Plus 594
- APC, APC-eFluor 780
- Brilliant UV 395, 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506, 570, 615, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, and mitosis) but absent in resting cells (G0). Ki-67 redistributes from the nucleus to chromosomes during mitosis and serves as a marker for cellular proliferation. The Ki-67 labeling index is used to predict outcomes in certain cancers. Ki-67 interacts with chromobox protein homolog 3 (CBX3).
The SolA15 antibody also recognizes human, non-human primate, and canine Ki-67.
Applications Reported:
- Intracellular staining and flow cytometric analysis
- Immunohistochemistry (frozen and paraffin-embedded tissue)
- Microscopy and immunocytochemistry
Applications Tested:
- Immunocytochemistry of fixed and permeabilized C2C12 cells (≤ 5 µg/mL)
- Intracellular staining and flow cytometry of stimulated mouse splenocytes (≤ 0.125 µg/test) using Foxp3/Transcription Factor Buffer Set (Product #00-5523-00)
- Refer to BestProtocols® for intracellular staining of nuclear proteins (Protocol B)
- Antibody should be titrated for optimal assay performance
- 0.2 µm post-manufacturing filtered
Target Information
Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin through its FHA domain and shows cell cycle–dependent localization: perinucleolar at G1, nuclear matrix at G2, and chromosomal during M phase. Ki-67 is widely used as a proliferation marker since it is absent in G0 cells. It is useful in determining the growth fraction of neoplasms and correlates with tumor grade and prognosis. Ki-67 is also used as a neuronal marker of cell proliferation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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