Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), NovaFluor Yellow 660, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: ≤ 0.8 µg/test

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 532, APC, APC-eFluor 780, Biotin, Brilliant UV 395/496/615/737/805, Brilliant Violet 711/786, eFluor 450, FITC, Functional Grade, NovaFluor Blue 510/610-30S/Red 685/Red 710/Yellow 570/Yellow 590, PE, PE-Cyanine5/7, PE-eFluor 610, PerCP-Cyanine5.5, PerCP-eFluor 710, Super Bright 436/600/645.
Custom conjugation available.

Product Specific Information

The M1/69 monoclonal antibody reacts with mouse CD24 (Heat Stable Antigen, HSA), a 35–50 kDa GPI-anchored membrane glycoprotein expressed on erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage cells. CD24 expression varies by lineage and is useful for resolving B lymphopoiesis stages in mouse bone marrow. CD24 has been reported to bind P-selectin (CD62P).

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes and thymocytes at ≤ 0.8 µg/test (100 µL sample). Optimal titration recommended.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• Always use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining. Add 5 µL per sample or per 100 µL cell suspension.
• NovaFluor conjugates utilize Phiton™ technology for engineered fluorescent signatures with minimized spillover and spread.

Excitation: 552 nm
Emission: 663 nm
Laser: 561 nm (Yellow Laser)

Note: NovaFluor Yellow 660 may cause non-specific staining of B cells in SJL mice. Not observed in BALB/c or C57BL/6 strains.

Target Information

CD24 is a GPI-anchored glycoprotein expressed throughout B-cell development (pro-B to mature B cells) but low or absent in plasma cells. It is also found in B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas. CD24 is implicated in regulating B-cell proliferation, maturation, and autoimmunity control.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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