Thermo Fisher Scientific BID Monoclonal Antibody (3C5)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA5-17034 targets BID in indirect ELISA, FACS, ICC, IHC, IF, and WB applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of human BID expressed in E. coli.
MA5-17034 detects BID with a predicted molecular weight of approximately 22 kDa.

Target Information

BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. It belongs to the BCL-2 family of cell death regulators. BID mediates mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves BID, and the COOH-terminal fragment translocates to mitochondria where it triggers cytochrome c release.
BID is a pro-apoptotic member of the BCL-2 family, normally existing in an inactive cytosolic form. Upon activation by caspase-8 in response to TNF-α or Fas ligand, BID is cleaved, and the C-terminal 15 kDa fragment (p15) translocates to mitochondria, initiating apoptosis.
Thus, BID serves as a direct molecular link between caspase-8 activation and mitochondrial death machinery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.