Thermo Fisher Scientific BID Monoclonal Antibody (3C5)
Human BID 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry, ELISA 등에 사용 가능. 비결합형 액상 형태이며, 4°C 또는 -20°C에서 보관. 세포사멸 관련 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3C5 |
| Immunogen | Purified recombinant full-length human BID protein expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2538506 |
Product Specific Information
MA5-17034 targets BID in indirect ELISA, FACS, ICC, IHC, IF, and WB applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of human BID expressed in E. coli.
MA5-17034 detects BID with a predicted molecular weight of approximately 22 kDa.
Target Information
BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. It belongs to the BCL-2 family of cell death regulators. BID mediates mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves BID, and the COOH-terminal fragment translocates to mitochondria where it triggers cytochrome c release.
BID is a pro-apoptotic member of the BCL-2 family, normally existing in an inactive cytosolic form. Upon activation by caspase-8 in response to TNF-α or Fas ligand, BID is cleaved, and the C-terminal 15 kDa fragment (p15) translocates to mitochondria, initiating apoptosis.
Thus, BID serves as a direct molecular link between caspase-8 activation and mitochondrial death machinery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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