Thermo Fisher Scientific Survivin Monoclonal Antibody (1H5)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA5-17035 targets BIRC5 in indirect ELISA, FACS, ICC, IHC, IF, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human BIRC5 expressed in E. coli.
MA5-17035 detects BIRC5, which has a predicted molecular weight of approximately 16 kDa.

Target Information

Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cellular functions such as signaling, division, and metabolism, and show differential expression in nearly all human cancers but not in most normal tissues.

IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain and lacks a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors but low in adult tissues.

Survivin is expressed in the G2/M phase of the cell cycle and associates with microtubules of the mitotic spindle in a regulated manner. Disruption of survivin-microtubule interactions leads to loss of anti-apoptotic function and increased caspase-3 activity during mitosis.

Survivin is also expressed in brain tissues (astrocytes and some neurons) following traumatic brain injury and may regulate neural cell proliferation. At least four transcript variants encoding distinct isoforms have been identified. Overexpression of survivin in cancer may overcome apoptotic checkpoints and favor aberrant mitotic progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.