Thermo Fisher Scientific CD31/PECAM-1 (Endothelial Cell Marker) Monoclonal Antibody (PECAM1/3530)
CD31/PECAM-1 단일클론 항체로 인간 내피세포 마커 검출에 적합. Western blot, IHC 등 다양한 응용 가능. 고순도 Protein A/G 정제, 보존제 무첨가 PBS 용액 형태. 연구용으로만 사용.
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Thermo Fisher Scientific CD31/PECAM-1 (Endothelial Cell Marker) Monoclonal Antibody (PECAM1/3530)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PECAM1/3530 |
| Immunogen | Recombinant fragment (around aa 625–738) of human CD31 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | –20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
Target Information
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling through dual immunoreceptor tyrosine-based inhibitory motifs (ITIMs). Upon phosphorylation by associated kinases, these motifs provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment, mainly at junctions between adjacent cells. Its N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions.
CD31 plays diverse roles in:
- Integrin-mediated cell adhesion
- Transendothelial migration
- Angiogenesis and apoptosis
- Negative regulation of immunoreceptor signaling
- Autoimmunity and macrophage phagocytosis
- IgE-mediated anaphylaxis and thrombosis
It is a key regulatory molecule in the vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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