Thermo Fisher Scientific CD31/PECAM-1 (Endothelial Cell Marker) Monoclonal Antibody (PECAM1/3534)
CD31/PECAM-1 단일클론 항체로 혈관 내피세포 표지에 사용. 인간 시료 반응성이며 WB, IHC, ICC, Flow 등 다양한 응용 가능. 고순도 Protein A/G 정제, 액상 형태로 안정적. 연구용 전용 시약.
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Thermo Fisher Scientific CD31/PECAM-1 (Endothelial Cell Marker) Monoclonal Antibody (PECAM1/3534)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PECAM1/3534 |
| Immunogen | Recombinant fragment (around aa 625–738) of human CD31 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling via dual immunoreceptor tyrosine-based inhibitory motifs (ITIM). Upon phosphorylation, these motifs serve as docking sites for protein-tyrosine phosphatases. CD31 is expressed throughout the vascular compartment, mainly at junctions between adjacent cells. Its N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions. CD31 plays diverse roles in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immune regulation, autoimmunity, phagocytosis, and thrombosis, making it a key regulatory molecule in the vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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