Thermo Fisher Scientific HBEGF Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human HB-EGF. Anti-Human HB-EGF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human HB-EGF by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human HB-EGF (500-P329) as a capture antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human HB-EGF.

Western Blot:
To detect Human HB-EGF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
When used with compatible development reagents, the detection limit for Recombinant Human HB-EGF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information:
500-P329BT-1MG will be provided as 2 × 500 µg.

Target Information

HBEGF is a growth factor that mediates its effects via EGFR, ERBB2, and ERBB4. It is required for normal cardiac valve formation and heart function.
HBEGF promotes smooth muscle cell proliferation and may be involved in macrophage-mediated cellular proliferation.
It is mitogenic for fibroblasts but not endothelial cells. It binds EGFR with higher affinity than EGF and is a potent mitogen for smooth muscle cells.
It also functions as a diphtheria toxin receptor.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.