Thermo Fisher Scientific IFN beta Polyclonal Antibody, PeproTech
Recombinant Human IFN-beta에 특이적인 Rabbit Polyclonal Antibody로, Western blot, ELISA 등 다양한 분석에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관합니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 500-P32B-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IFN beta Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | – | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 2 publications |
| In vitro Assay (IV) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IFN-beta |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930446 |
Product Specific Information
AA Sequence of recombinant protein:
MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IFN-beta. Anti-Human IFN-beta-specific antibody was purified by affinity chromatography employing an immobilized Human IFN-beta matrix.
Sandwich ELISA:
To detect Human IFN-beta by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IFN-beta (500-P32BBt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human IFN-beta.
Western Blot:
To detect Human IFN-beta by Western Blot, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, detection limit for Recombinant Human IFN-beta is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit tumor development. IFN-beta binding activates tyrosine kinases Jak1 and Tyk2, phosphorylates STAT transcription factors, and induces immune response gene expression. Members of the toll-like receptor (TLR) family also stimulate IFN-beta production. Clinically, IFN-beta is used for treatment of tumors, infections, and multiple sclerosis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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