Thermo Fisher Scientific LIGHT Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MRLHQRLGDI VAHLPDGGKG SWEKLIQDQR SHQANPAAHL TGANASLIGI GGPLLWETRL GLAFLRGLTY HDGALVTMEP GYYYVYSKVQ LSGVGCPQGL ANGLPITHGL YKRTSRYPKE LELLVSRRSP CGRANSSRVW WDSSFLGGVV HLEAGEEVVV RVPGNRLVRP RDGTRSYFGA FMV

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine LIGHT. Anti-Murine LIGHT-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Murine LIGHT by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine LIGHT (500-P308) as a capture antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Murine LIGHT.

Western Blot:
To detect Murine LIGHT by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Murine LIGHT is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P308BT-1MG is provided as 2 × 500 µg.

Target Information

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family.

A novel DD-containing molecule, Daxx (death associated protein 6), binds specifically to the Fas death domain and enhances Fas-induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues, indicating its important function in Fas signaling pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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