Thermo Fisher Scientific Ly-6D Monoclonal Antibody (49-H4), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2c, kappa

Class

Monoclonal

Type

Antibody

Clone

49-H4

Conjugate

PE PE PE

View additional formats

• eFluor 450
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11042719

Product Specific Information

Description: This 49-H4 monoclonal antibody reacts with mouse Ly-6D, which is also known as ThB. This member of the Ly6 family of GPI-anchored cell surface glycoproteins is expressed on thymocytes and splenic B cells. Moreover, common lymphoid progenitor cells that express Ly-6D have been shown to commit to the B cell lineage. Expression of Ly-6D has also been associated with Rag-1 expression along with a reduced ability to differentiate into T or NK cells. Finally, reports indicate that the level of Ly-6D expression varies in different mouse strains.

Applications Reported: This 49-H4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 49-H4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

May act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. Marks the earliest stage of B-cell specification. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.