Thermo Fisher Scientific Methyl-PP2A alpha (Leu309) Monoclonal Antibody (2A10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2A10

Immunogen

A KLH-coupled peptide with the sequence: KLH-C-ßA-RRTPDYFL-OMe (aa 302-309). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PP2A alpha,uniProtId:P67775-1,ncbiNodeId:9606,antigenRange:302-309,antigenLength:309,antigenImageFileName:MA5-18072_PP2A_alpha_P67775-1_House_mouse.svg,antigenImageFileNamePDP:MA5-18072_PP2A_alpha_P67775-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539455

Product Specific Information

This antibody recognizes the C-terminal methylated form of PP2A (C subunit). The c-terminal sequence, as well as the carboxy-methylation, is 100% conserved in human, mouse and yeast.

Western blot results show a band at 36 kDa, which matches the predicted molecular weight.

The c-terminal sequence (as well as the carboxy-methylation) is absolutely conserved from human, mouse to yeast.

Target Information

In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2A alpha and PP2A beta. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.