Thermo Fisher Scientific H4K20me3 Monoclonal Antibody (6F8-D9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:4,000

-

ELISA (ELISA)

Assay-dependent

-

ChIP assay (ChIP)

Assay-dependent

-

Dot blot (DB)

Assay-dependent

-

Product Specifications

Species Reactivity

C. elegans, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6F8-D9

Immunogen

Synthetic peptide of Human Histone H4 (Immunogen sequence: 15-AKRHR(Kme3)VLRDN-25 (branched)) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H4K20me3,uniProtId:P62805-1,ncbiNodeId:9606,antigenRange:15-25|16-20,antigenLength:103,antigenImageFileName:MA5-18074_H4K20me3_P62805-1_House_mouse.svg,antigenImageFileNamePDP:MA5-18074_H4K20me3_P62805-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539457

Product Specific Information

This antibody shows weak cross-reactivity with K20 di-methylated histone H4, but does not react with histone H3 or mono- or unmethylated K20 histone 4. This antibody is predicted to react with Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogaster), and mammals.

This target has a predicted molecular weight of 11 kDa.

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.