Thermo Fisher Scientific H4K20me3 Monoclonal Antibody (6F8-D9)
H4K20me3 단일클론 항체(6F8-D9)는 인간 및 C. elegans 시료에서 사용 가능하며, Western blot, ELISA, ChIP, Dot blot에 적합합니다. H4 K20 삼중메틸화 히스톤에 특이적이며 단백질 G로 정제된 액상 형태의 항체입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:4,000 | - |
| ELISA | Assay-dependent | - |
| ChIP assay | Assay-dependent | - |
| Dot blot (DB) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | C. elegans, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6F8-D9 |
| Immunogen | Synthetic peptide of Human Histone H4 (sequence: 15-AKRHR(Kme3)VLRDN-25 (branched)) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C (avoid freeze/thaw cycles). |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539457 |
Product Specific Information
This antibody shows weak cross-reactivity with K20 di-methylated histone H4, but does not react with histone H3 or mono- or unmethylated K20 histone 4.
Predicted to react with Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, and mammals.
Predicted molecular weight: 11 kDa
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of eukaryotic chromatin. Two molecules each of H2A, H2B, H3, and H4 form an octamer around which ~146 bp of DNA is wrapped, forming nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes to compact chromatin into higher-order structures.
The H4 gene is intronless, encodes a histone H4 family member, and contains a palindromic termination element instead of a polyA tail. It is located in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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