Thermo Fisher Scientific CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), NovaFluor Blue 660-120S

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.6 µg/test

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Product Specifications

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Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 555
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

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Product Specific Information

The eBioMWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin alpha IIb). CD41 is expressed on platelets, megakaryocytes, and hematopoietic progenitors. It forms a receptor complex with CD61, mediating platelet adhesion and aggregation via fibrinogen, fibronectin, and vitronectin binding.

Recent studies show CD150+CD48−CD41− profiles efficiently identify hematopoietic stem cells (HSCs).

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometry of mouse platelets (≤0.125 µg/test). Optimize antibody concentration empirically.

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) should be used for optimal staining.
  • Add 5 µL per sample before mixing with cells.
  • For single-color controls, use 5 µL per 100 µL sample containing 10³–10⁸ cells.

NovaFluor conjugates utilize Phiton™ technology based on nucleic acid dye structures engineered for minimal spillover and spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

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Target Information

CD41 (platelet glycoprotein IIb, ITGA2B) consists of α (120 kDa) and β (23 kDa) subunits that interact with CD61 in the presence of calcium to form a functional adhesive receptor. It mediates platelet aggregation and binds von Willebrand factor, fibrinogen, fibronectin, and vitronectin. CD41 is mainly expressed in the megakaryocyte-platelet lineage and during early hematopoietic differentiation.
Diseases associated with CD41 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 bleeding disorder.

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For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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