Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), Super Bright 436, eBioscience
마우스 CD31(PECAM-1)을 인식하는 단클론 항체로, Super Bright 436 형광체로 결합됨. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 0.25 µg/test 권장. 바이올렛 레이저(405 nm)로 여기, 436 nm에서 방출. 혈관 및 면역세포 연구용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunohistochemistry (IHC) | - | 13 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 |
| Immunocytochemistry (ICC/IF) | - | 10 |
| Flow Cytometry (Flow) | 0.25 µg/test | 46 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 390 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802404 |
Available Formats
- Unconjugated
- APC
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 711
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 610
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The 390 monoclonal antibody reacts with mouse CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed by endothelial cells and at low levels on all leukocytes and platelets. CD31 mediates homotypic interactions important for adhesion, cell-cell and cell-matrix interactions, and signal transduction.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤0.25 µg/test in 100 µL final volume. Cell number range: 10⁵–10⁸ cells/test. Titrate for optimal performance.
Fluorochrome Information:
Super Bright 436 is excited with the 405 nm violet laser and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling via ITIM motifs, providing docking sites for protein-tyrosine phosphatases. It is expressed throughout the vascular system, mainly at cell junctions. The N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions. CD31 plays roles in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immune regulation, phagocytosis, anaphylaxis, and thrombosis.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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