Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), Super Bright 436, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 711
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The 390 monoclonal antibody reacts with mouse CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed by endothelial cells and at low levels on all leukocytes and platelets. CD31 mediates homotypic interactions important for adhesion, cell-cell and cell-matrix interactions, and signal transduction.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤0.25 µg/test in 100 µL final volume. Cell number range: 10⁵–10⁸ cells/test. Titrate for optimal performance.

Fluorochrome Information:
Super Bright 436 is excited with the 405 nm violet laser and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling via ITIM motifs, providing docking sites for protein-tyrosine phosphatases. It is expressed throughout the vascular system, mainly at cell junctions. The N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions. CD31 plays roles in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immune regulation, phagocytosis, anaphylaxis, and thrombosis.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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