Thermo Fisher Scientific SIGLEC H Monoclonal Antibody (eBio440c), Super Bright 436, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test
• Publications: View 3 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• Brilliant UV 615
• eFluor 450
• eFluor 660
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on plasmacytoid dendritic cells (pDCs) or type I IFN-producing cells (IPC) in naive mice.
Mouse IPCs are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are rapid responders to viral infections.

Siglec-H is a transmembrane protein of the Ig superfamily that binds sialic acid like CD33 but lacks a cytoplasmic ITIM domain. Instead, it associates with DAP12 to enable signal transduction.
The eBio440c antibody has been shown to inhibit pDC function by reducing IFNα secretion in response to CpG.

Some mouse strains (e.g., SJL) display higher percentages of pDCs compared to C57BL/6.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test (100 µL final volume)
• Excitation: 405 nm
• Emission: 436 nm
• Laser: Violet laser

For optimal performance, titrate antibody concentration for each assay.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Siglec-H is a transmembrane protein found on pDCs or type I IFN-producing cells in naive mice.
It binds sialic acid and associates with DAP12 for signal transduction.
The eBio440c antibody inhibits pDC function and IFNα secretion upon CpG stimulation.
Certain mouse strains (e.g., SJL) exhibit higher pDC levels than C57BL/6.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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