Thermo Fisher Scientific DYKDDDDK Tag Chimeric Recombinant Mouse Monoclonal Antibody (8H8L17)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Flow Cytometry (Flow)

0.25 µg/test

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG

Expression System

ExpiCHO

Class

Recombinant Monoclonal

Type

Antibody

Clone

8H8L17

Immunogen

Synthetic peptide (DYKDDDDK)

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Alexa Fluor Plus 594
• Alexa Fluor Plus 647
• Biotin
• HRP
• Request custom conjugation

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

This antibody has Rabbit Variable heavy and light chains, and Mouse constant domains. It recognizes c-terminal DYKDDDDK tag. This chimeric antibody is recognized by anti-mouse secondary antibodies and has no cross reactivity to anti-rabbit secondary antibodies.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.