Thermo Fisher Scientific DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

8H8L17

Immunogen

Synthetic peptide (DYKDDDDK)

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Alexa Fluor Plus 594
• Alexa Fluor Plus 647
• HRP
• Request custom conjugation

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942239

Product Specific Information

DYKDDDDK Tag recombinant rabbit monoclonal antibody conjugated with Biotin specifically recognizes DYKDDDDK Tag at the C-terminus but not N-terminus of proteins.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity, lot-to-lot consistency, and broader immunoreactivity to diverse targets due to a larger rabbit immune repertoire.

This antibody has been tested in western blot. The clone is reported to also work in immunoprecipitation (IP) assay, and it is recommended that the antibody be carefully titrated for optimal performance in this assay for best results.

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.