Thermo Fisher Scientific DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17), Biotin

Applications

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Alexa Fluor Plus 594
• Alexa Fluor Plus 647
• HRP
• Request custom conjugation

Product Specific Information

DYKDDDDK Tag recombinant rabbit monoclonal antibody conjugated with Biotin specifically recognizes the DYKDDDDK Tag at the C-terminus but not the N-terminus of proteins.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These antibodies are derived from specific genes encoding the desired antibodies, cloned into expression vectors, and expressed in vitro.
Advantages include:

• Enhanced specificity
• Lot-to-lot consistency
• Broader immunoreactivity due to the rabbit immune repertoire

This antibody has been validated for western blot and is reported to work in immunoprecipitation (IP). It is recommended to titrate the antibody for optimal IP performance.

Target Information

The DYKDDDDK tag (also known as FLAG tag) is a short peptide sequence used for protein purification, detection, and localization.

• Sequence: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
• Can be fused to N- or C-terminus of target proteins
• Enables easy identification and isolation using anti-FLAG antibodies

The DYKDDDDK tag is widely used in molecular biology and biochemistry for:

• Protein-protein interaction studies
• Protein expression analysis
• Protein localization studies

The FLAG tag is more hydrophilic than other epitope tags, minimizing protein denaturation or inactivation.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.