Thermo Fisher Scientific Phospho-Connexin 43 (Ser279) Polyclonal Antibody

Applications

• Western Blot (WB): 1:1,000–1:3,000

Product Specifications

Product Specific Information

This antibody detects endogenous levels of Connexin 43 (GJA1) only when phosphorylated at Ser279.

Target Information

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins form hexamers that are transported to the plasma membrane to create hemichannels, which pair with those on adjacent cells to form gap junctions. These structures enable direct intercellular communication, crucial for development, cell growth regulation, and cardiac synchronization.

Phosphorylation of Cx43 regulates gap junction assembly and function. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC), reducing cell-to-cell communication. Src kinase can also phosphorylate Cx43, altering its communication properties. Connexin 43 is the predominant gap junction protein in the heart, and mutations or dysregulation can lead to cardiac and developmental abnormalities. A related pseudogene, GJA1P, is located on chromosome 5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.