Thermo Fisher Scientific TFRC Monoclonal Antibody (OTI6H9), TrueMAB

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI6H9

Immunogen

Full length human recombinant protein of human TFRC produced in HEK293T cell. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Transferrin Receptor (CD71),uniProtId:P02786-1,ncbiNodeId:9606,antigenRange:1-760,antigenLength:760,antigenImageFileName:CF803640_Transferrin_Receptor_CD71_P02786-1_House_mouse.svg,antigenImageFileNamePDP:CF803640_Transferrin_Receptor_CD71_P02786-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.