Thermo Fisher Scientific beta-5 Tubulin Recombinant Rabbit Monoclonal Antibody (SR25-04)
베타-5 튜불린을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry에 적합. 높은 특이성과 재현성을 제공하며, HEK293 세포에서 발현된 단백질 A 정제 항체. 연구용으로 세포 구조 및 미세소관 분석에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:400 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SR25-04 |
| Immunogen | Synthetic peptide within Human beta Tubulin (aa 308–357) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809302 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal clones for production. Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Microtubules are major cytoskeletal components in eukaryotic cells, composed of tubulin dimers (alpha and beta subunits, each ~55 kDa). They are essential for:
- Chromosome segregation during mitosis
- Intracellular transport
- Ciliary and flagellar motility
- Structural support of the cytoskeleton
This antibody does not induce filament aggregation or block microtubule assembly. It does not inhibit tubulin polymerization/depolymerization and partially blocks the ability of tubulin dimers (with GppNHp bound) to inhibit adenylyl cyclase.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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