Thermo Fisher Scientific CD184 (CXCR4) Monoclonal Antibody (2B11), eFluor 450, eBioscience

Thermo Fisher Scientific CD184 (CXCR4) Monoclonal Antibody (2B11), eFluor 450

Applications & Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 805
• Functional Grade
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation available

Product Specific Information

The 2B11 monoclonal antibody reacts with mouse and human CXCR4 (CD184), also known as Fusin, LESTR, or HUMSTR.
CXCR4 is a G-protein-coupled chemokine receptor involved in HIV-1 entry into CD4+ cells and lymphocyte migration.
Its ligand is SDF1, essential for B cell development and granulocyte progenitor maintenance.
Expression differs between species — in mice, CXCR4 is mainly expressed on CD4+CD8+ thymocytes and B lymphocytes in the spleen.

The 2B11 antibody recognizes the amino terminus of CXCR4 and induces receptor internalization without blocking SDF1a.
Tested for flow cytometric analysis of mouse thymocytes (≤1 µg/test).
Cell number range: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal assay performance.

eFluor™ 450 is an alternative to Pacific Blue™, excited by Violet laser (405 nm) and emits at 445 nm.
Ensure instrument compatibility for detection.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a G-protein-coupled receptor with seven transmembrane domains.
Functions as co-receptor for HIV-1 and HIV-2 entry into CD4+ cells via gp120-mediated fusion.
Acts as receptor for CXCL12/SDF-1, mediating calcium influx and MAPK activation.
Also serves as receptor for extracellular ubiquitin, influencing cAMP levels.
Involved in hematopoiesis, cardiac septum formation, and vascular remodeling.
Mediates hippocampal neuron survival and cerebellar development.
Binds bacterial LPS and triggers inflammatory responses, including TNF secretion.
Antibodies to CXCR4 block HIV fusion and infection of human cells.
The amino-terminal domain and second extracellular loop serve as HIV binding sites.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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