Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), eFluor 450, eBioscience

Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
• Brilliant Violet 480 / 605 / 650 / 786
• eFluor 506
• FITC
• Functional Grade
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is mainly expressed on activated T and B lymphocytes.

Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). J43 inhibits the binding of mouse PD-L1-Ig and PD-L2-Ig to PD-1/BHK transfected cells. In vivo, J43 (intact or Fab) enhances contact hypersensitivity and acute GVHD, similar to PD-1-deficient cell transfer. Injection of J43 also exacerbates EAE and NOD diabetes.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry of stimulated mouse splenocytes at ≤0.5 µg/test.
Recommended use: Titrate carefully for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.

Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm (Violet laser compatible).

Target Information

PD-1 (PDCD-1) mediates inhibitory signals in activated T cells through binding to PD-L1 and PD-L2. This interaction limits immune activation in absence of danger signals (e.g., LPS). PD-1 knockout mice show hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at a higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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