Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), eFluor 450, eBioscience
마우스 PD-1 단백질을 인식하는 J43 클론의 단클론 항체로, eFluor 450 형광체가 결합된 제품입니다. Flow cytometry에 최적화되어 있으며, 면역관용 및 자가면역 연구에 활용됩니다. 405/445 nm 파장에서 검출되며, 4°C 암소 보관이 필요합니다.
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Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 36 publications |
| Functional Assay (Functional) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | J43 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574138 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 480 / 605 / 650 / 786
- eFluor 506
- FITC
- Functional Grade
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is mainly expressed on activated T and B lymphocytes.
Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). J43 inhibits the binding of mouse PD-L1-Ig and PD-L2-Ig to PD-1/BHK transfected cells. In vivo, J43 (intact or Fab) enhances contact hypersensitivity and acute GVHD, similar to PD-1-deficient cell transfer. Injection of J43 also exacerbates EAE and NOD diabetes.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry of stimulated mouse splenocytes at ≤0.5 µg/test.
Recommended use: Titrate carefully for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.
Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm (Violet laser compatible).
Target Information
PD-1 (PDCD-1) mediates inhibitory signals in activated T cells through binding to PD-L1 and PD-L2. This interaction limits immune activation in absence of danger signals (e.g., LPS). PD-1 knockout mice show hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at a higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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