Thermo Fisher Scientific COL1A2 Monoclonal Antibody (4D1A7)
COL1A2 단백질을 인식하는 Mouse monoclonal antibody (Clone 4D1A7). Western blot, ICC/IF, Flow cytometry에 적합. Protein G로 정제된 액상 항체로 1 mg/mL 농도. 인간 COL1A2 단백질(aa 23-79)에서 유래된 면역원 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4D1A7 |
| Immunogen | Purified recombinant fragment of human COL1A2 (aa: 23–79) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898416 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
The COL1A2 gene encodes the pro-alpha2 chain of type I collagen, which forms a triple helix with two alpha1 chains and one alpha2 chain. Type I collagen is a fibril-forming collagen abundant in bone, cornea, dermis, and tendon.
Mutations in this gene are associated with several connective tissue disorders, including:
- Osteogenesis imperfecta types I–IV
- Ehlers-Danlos syndrome type VIIB and classical type
- Recessive Ehlers-Danlos syndrome
- Idiopathic osteoporosis
- Atypical Marfan syndrome
Symptoms from COL1A2 mutations are generally less severe than those from COL1A1 mutations, reflecting the differing roles of alpha2 chains in matrix integrity.
Three transcripts have been identified, resulting from alternate polyadenylation signal usage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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