Thermo Fisher Scientific DLAT Recombinant Rabbit Monoclonal Antibody (ARC1314)
DLAT 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot 및 ELISA에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며, HEK293 세포에서 발현되었습니다. 고순도 친화 크로마토그래피 정제 및 안정한 PBS/glycerol 버퍼로 보관됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1314 |
| Immunogen | Synthetic peptide corresponding to amino acids 548–647 of human DLAT (P10515) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS (pH 7.3) with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849623 |
Product Specific Information
Immunogen sequence:
AREGKLQPHE FQGGTFTISN LGMFGIKNFS AIINPPQACI LAIGASEDKL VPADNEKGFD VASMMSVTLS CDHRVVDGAV GAQWLAEFRK YLEKPITMLL
Target Information
DLAT encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC).
PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A.
The protein product, dihydrolipoamide acetyltransferase, transfers acetyl groups to coenzyme A.
Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies, which are present in nearly 95% of patients with primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy bile duct epithelial cells where DLAT is abnormally distributed and overexpressed, leading eventually to cirrhosis and liver failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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