Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Biotin, eBioscience

Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• APC
• Brilliant UV 615 / 661 / 737 / 805
• Brilliant Violet 421 / 650 / 786
• Functional Grade
• NovaFluor Red 700 / 755
• NovaFluor Yellow 690 / 700
• PE / PE-Cyanine7 / PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 780
• Request custom conjugation

Product Specific Information

The MIH5 monoclonal antibody reacts with mouse B7-H1 (PD-L1). B7-H1 is a member of the B7 family with a predicted molecular weight of ~40 kDa and belongs to the Ig superfamily. It is expressed on T, B, NK, and dendritic cells and acts as a ligand for PD-1. The PD-1/PD-L1 interaction inhibits T and B cell responses. MIH5 is reported to be a blocking antibody.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.25 µg/test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Antibody titration recommended for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Programmed death receptor ligand 1 (PD-L1, B7-H1) is a B7 family member that binds to PD-1, negatively regulating T-cell receptor (TCR) signaling. PD-L1 ligation decreases TCR-mediated proliferation and cytokine production. PD-L1 expression is abundant in murine and human cancers and upregulated by IFN-γ stimulation, suggesting its role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.