Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Biotin, eBioscience
Mouse PD-L1(B7-H1) 단백질을 인식하는 MIH5 클론의 Biotin 결합 단일클론 항체입니다. Flow Cytometry에 최적화되어 있으며 세포막 PD-L1 검출 및 면역억제 경로 연구에 활용됩니다. 고순도 정제, 4°C 보관, 연구용 전용 시약입니다.
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Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 8 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 38 publications |
| Neutralization (Neu) | - | View 5 publications |
| Functional Assay (Functional) | - | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, lambda |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MIH5 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466836 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 660
- APC
- Brilliant UV 615 / 661 / 737 / 805
- Brilliant Violet 421 / 650 / 786
- Functional Grade
- NovaFluor Red 700 / 755
- NovaFluor Yellow 690 / 700
- PE / PE-Cyanine7 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 780
- Request custom conjugation
Product Specific Information
The MIH5 monoclonal antibody reacts with mouse B7-H1 (PD-L1). B7-H1 is a member of the B7 family with a predicted molecular weight of ~40 kDa and belongs to the Ig superfamily. It is expressed on T, B, NK, and dendritic cells and acts as a ligand for PD-1. The PD-1/PD-L1 interaction inhibits T and B cell responses. MIH5 is reported to be a blocking antibody.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.25 µg/test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Antibody titration recommended for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Programmed death receptor ligand 1 (PD-L1, B7-H1) is a B7 family member that binds to PD-1, negatively regulating T-cell receptor (TCR) signaling. PD-L1 ligation decreases TCR-mediated proliferation and cytokine production. PD-L1 expression is abundant in murine and human cancers and upregulated by IFN-γ stimulation, suggesting its role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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