Thermo Fisher Scientific Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Biotin, eBioscience
Mouse Sca-1(Ly-6A/E) 인식 단클론 항체 D7, Biotin 결합형. Flow cytometry에 최적화되어 있으며, hematopoietic stem cell 및 T/B 세포 활성 연구에 활용. 0.5 µg/test 농도로 사용 권장, 4°C 보관.
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Thermo Fisher Scientific Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Biotin, eBioscience™
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 8 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 5 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 90 publications |
| Miscellaneous (PubMed) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | D7 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466834 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- Brilliant UV 395
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 711
- Brilliant Violet 786
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins.
D7 recognizes both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid populations, and peripheral T and B cells.
Non-activated splenocytes from Ly-6.2 strains (C57BL, SJL, 129, AKR, etc.) show higher frequency of D7-positive cells compared to Ly-6.1 strains (BALB/c, C3H, NZB, etc.).
Sca-1 expression increases upon activation regardless of Ly-6 haplotype.
Sca-1 serves as a major phenotypic marker for mouse hematopoietic progenitor/stem cell subsets.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse bone marrow cells, ≤0.5 µg/test.
A test is defined as the amount of antibody that stains a cell sample in 100 µL final volume (10⁵–10⁸ cells/test).
Antibody should be titrated for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family.
It is expressed on multipotent hematopoietic stem cells (HSC) and used as a marker of HSC in mice.
Sca-1 is used with lineage depletion antibodies to identify murine HSC and is found in fetal liver, adult bone marrow, peripheral blood, and spleen.
It may be involved in regulation of B and T cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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