Thermo Fisher Scientific CD202b (TIE2) Monoclonal Antibody (TEK4), Biotin, eBioscience
Mouse Tie-2 (CD202b) 인식 단클론 항체로, Biotin으로 결합된 제품입니다. Flow cytometry 및 다양한 IHC 응용에 적합하며, 0.5 µg/test로 사용 가능합니다. 혈관형성 및 조혈 관련 연구에 활용되며, 4°C에서 암실 보관합니다.
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Thermo Fisher Scientific CD202b (TIE2) Monoclonal Antibody (TEK4), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 28 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TEK4 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466847 |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
Product Specific Information
The TEK4 monoclonal antibody reacts with mouse Tie-2 (CD202), a tyrosine kinase receptor expressed on endothelial and certain hematopoietic cells. Tie-2 plays a role in angiogenesis and hematopoiesis during mouse embryonic development.
In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells (Lineage^-, c-Kit^+, Sca1^+). Long-term multilineage repopulating cells were detected in Tie-2^+, Lineage^-, c-Kit^+, Sca1^+ cells but not in Tie-2^- cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤ 0.5 µg/test (100 µL final volume). Cell number may range from 10^5–10^8 cells/test. Antibody titration recommended for optimal performance.
Target Information
The TEK (TIE2) receptor tyrosine kinase is mainly expressed in endothelial cells and is essential for endothelial–smooth muscle cell communication in venous morphogenesis. Defects in TEK are linked to inherited venous malformations.
Angiopoietin-1 binds to TEK and mediates signaling crucial for embryonic vascular development. TIE2 expression increases with thyroid-stimulating hormone and agents elevating intracellular cAMP.
Hematopoietic stem cells (HSCs) expressing TIE2 are quiescent, antiapoptotic, and adhere to osteoblasts in the bone marrow niche.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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