Thermo Fisher Scientific CD8a Monoclonal Antibody (4SM16), eBioscience
마우스 CD8a를 인식하는 단클론 항체로, IHC(FFPE 및 동결 조직) 분석에 적합합니다. Rat IgG2a, kappa 아이소타입이며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 친화 크로마토그래피로 정제되어 높은 순도와 재현성을 보장합니다.
- 카탈로그번호
- 14-0195-xx (4개 옵션)
- 판매단위
- pk
카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD8a Monoclonal Antibody (4SM16), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 11 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | View 3 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 5 µg/mL | View publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4SM16 |
| Immunogen | Fragment of extracellular domain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864836 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The 4SM16 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ heterodimers or CD8αα homodimers. CD8 binds to MHC class I and, through its association with protein tyrosine kinase p56lck, plays a key role in T cell development and activation.
This antibody is recommended for immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) or frozen mouse tissue and can also be used for staining mouse neural tissue.
Applications Reported:
Microscopy and immunohistochemical staining of frozen and FFPE tissue sections.
Applications Tested:
- Tested by IHC on FFPE mouse tissue using low/high pH antigen retrieval ≤ 5 µg/mL.
- Tested by IHC on frozen mouse tissue ≤ 5 µg/mL.
- Optimal performance requires titration per assay.
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein involved in signal transduction, T cell differentiation, and activation. CD8 primarily exists as a CD8αβ heterodimer acting as a co-receptor with TCR for MHC class I molecules. CD8 is also found as CD8αα homodimers on subsets of T cells, NK cells, monocytes, and dendritic cells. Engagement with MHC-I/peptide complexes recruits Lck kinase, inducing lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activation. Activated CTLs are crucial for pathogen and tumor cell clearance, with differentiation enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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