Thermo Fisher Scientific DCTN1 Monoclonal Antibody (1E12)
DCTN1 단백질을 인식하는 Mouse monoclonal antibody (Clone 1E12). Western blot 및 ELISA에 적합하며, 인간 시료에 반응. Affinity chromatography로 정제된 액상 시약으로, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| ELISA | 0.1 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1E12 |
| Immunogen | DCTN1 (AAH06163, 1 a.a. ~198 a.a) full-length recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
MPGPGLVKDS PLLLQQISAM RLHISQLQHE NSILKGAQMK ASLASLPPLH VAKLSHEGPG SELPAGALYR KTSQLLETLN QLSTHTHVVD ITRTSPAAKS PSAQLMEQVA QLKSLSDTVE KLKDEVLKET VSQRPGATVP TDFATFPSSA FLRAKEEQQD DTVYMGKVTF SCAAGFGQRH RLVLTQEQLH QLHSRLIS
Target Information
This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kDa. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in diverse cellular functions such as:
- ER-to-Golgi transport
- Centripetal movement of lysosomes and endosomes
- Spindle formation and chromosome movement
- Nuclear positioning and axonogenesis
This subunit interacts directly with the dynein intermediate chain and binds microtubules via a conserved CAP-Gly domain at its N-terminus. Alternative splicing produces multiple isoforms. Mutations in this gene are associated with distal hereditary motor neuronopathy type VIIB (HMN7B), also known as distal spinal and bulbar muscular atrophy (dSBMA).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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