Thermo Fisher Scientific DDB1 Monoclonal Antibody (4C5)
DDB1 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, Western blot, IHC, ICC/IF, ELISA에 적합합니다. 인간 시료 반응성이 있으며, PBS 버퍼에 보존된 액상 형태입니다. 연구용으로 DNA 손상 결합 단백질 분석에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.3 ng/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4C5 |
| Immunogen | DDB1 (NP_001914, 1044–1140 a.a.) partial recombinant protein with GST tag (MW of GST tag alone: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
SESWYNLLLD MQNRLNKVIK SVGKIEHSFW RSFHTERKTE PATGFIDGDL IESFLDISRP KMQEVVANLQ YDDGSGMKRE ATADDLIKVV EELTRIH
Target Information
The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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