Thermo Fisher Scientific Phospho-AKT1/AKT2/AKT3 (Ser473) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human AKT1(Accession P31749), corresponding to amino acid residues around phosphorylated Ser473. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AKT Pan,uniProtId:P31749-1,ncbiNodeId:9606,antigenRange:473,antigenLength:480,antigenImageFileName:PA5-104445_AKT_Pan_P31749-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104445_AKT_Pan_P31749-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:AKT Pan,uniProtId:Q9Y243-1,ncbiNodeId:9606,antigenRange:472|473,antigenLength:479,antigenImageFileName:PA5-104445_AKT_Pan_Q9Y243-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104445_AKT_Pan_Q9Y243-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816022

Product Specific Information

Antibody detects endogenous levels of pan-AKT1/2/3 only when phosphorylated at Sersine 473.

Target Information

AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.