Thermo Fisher Scientific Phospho-AKT1/AKT2/AKT3 (Ser473) Polyclonal Antibody
AKT1/2/3의 Ser473 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체입니다. Western blot, IHC, ICC/IF에 사용 가능하며, 다양한 종에 반응합니다. 순도 높은 액상 형태로 제공되며, -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human AKT1 (Accession P31749), corresponding to amino acid residues around phosphorylated Ser473. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816022 |
Product Specific Information
This antibody detects endogenous levels of pan-AKT1/2/3 only when phosphorylated at Serine 473.
Target Information
AKT, also known as protein kinase B (PKB) or RAS-alpha, is a serine/threonine kinase involved in regulating metabolism, cell survival, and growth. It is activated by insulin, PI3K, IGF1, and other growth factors. AKT promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of targets such as forkhead transcription factors and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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