Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), PerCP-Cyanine5.5, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), PerCP-Cyanine5.5, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.25 µg)/test
  View 62 publications

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 605
• eFluor 450 / 506 / 660
• FITC
• Functional Grade
• NovaFluor Blue 510 / 585
• NovaFluor Yellow 590 / 660 / 730
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. It has potent immunosuppressive properties and has been effective in treating renal, heart, and liver allograft rejection.
CD3 subunits (gamma, delta, epsilon) are essential for TCR complex assembly and surface expression. CD3 is expressed by thymocytes and mature T cells. Crosslinking of TCR initiates intracellular activation and proliferation pathways.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested by flow cytometry on normal human peripheral blood cells
  • Recommended use: 5 µL (0.25 µg)/test
  • Final volume: 100 µL
  • Cell number: 10^5–10^8 cells/test
• Excitation: 488 nm
• Emission: 695 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

The CD3 complex transduces antigen-recognition signals in T cells and regulates TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) are structurally related immunoglobulin family members encoded on human chromosome 11.
These subunits associate with signal transduction molecules via tyrosine-based motifs. CD3 may influence TCR-induced growth arrest, survival, and proliferation.
CD3 antigen is expressed on:

• 68–82% of peripheral blood lymphocytes
• 65–85% of thymocytes
• Purkinje cells in the cerebellum
  Not expressed on B or NK cells.
  Defects in CD3 genes are linked to CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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