Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), PerCP-Cyanine5.5, eBioscience
인간 CD3 복합체의 epsilon 서브유닛을 인식하는 OKT3 단클론 항체로, PerCP-Cyanine5.5 형광체가 결합되어 있습니다. Flow Cytometry에 최적화되어 있으며, 5 µL/test로 사용 가능합니다. 면역억제 특성을 가지며 T세포 활성화 연구에 적합합니다.
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Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), PerCP-Cyanine5.5, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PerCP-Cyanine5.5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT3 |
| Conjugate | PerCP-Cyanine5.5 |
| Excitation / Emission Max | 489 / 679 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10548354 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 605
- eFluor 450 / 506 / 660
- FITC
- Functional Grade
- NovaFluor Blue 510 / 585
- NovaFluor Yellow 590 / 660 / 730
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. It has potent immunosuppressive properties and has been effective in treating renal, heart, and liver allograft rejection.
CD3 subunits (gamma, delta, epsilon) are essential for TCR complex assembly and surface expression. CD3 is expressed by thymocytes and mature T cells. Crosslinking of TCR initiates intracellular activation and proliferation pathways.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and tested by flow cytometry on normal human peripheral blood cells
- Recommended use: 5 µL (0.25 µg)/test
- Final volume: 100 µL
- Cell number: 10^5–10^8 cells/test
- Excitation: 488 nm
- Emission: 695 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
The CD3 complex transduces antigen-recognition signals in T cells and regulates TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) are structurally related immunoglobulin family members encoded on human chromosome 11.
These subunits associate with signal transduction molecules via tyrosine-based motifs. CD3 may influence TCR-induced growth arrest, survival, and proliferation.
CD3 antigen is expressed on:
- 68–82% of peripheral blood lymphocytes
- 65–85% of thymocytes
- Purkinje cells in the cerebellum
Not expressed on B or NK cells.
Defects in CD3 genes are linked to CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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