Thermo Fisher Scientific CD3 Monoclonal Antibody (SK7), PerCP-Cyanine5.5, eBioscience

Applications

Immunohistochemistry (Frozen) (IHC (F))

• Not tested
• View 1 publication

Flow Cytometry (Flow)

• Recommended dilution: 5 µL (0.03 µg)/test
• View 11 publications

Product Specifications

Available Conjugate Formats

• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 661
• Brilliant Violet 650
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 690
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The SK7 monoclonal antibody reacts with human and chimpanzee CD3ε, a 20 kDa subunit of the TCR complex. Along with other CD3 subunits (γ and δ), the ε chain is essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. SK7 and UCHT1 clones cross-block binding, suggesting recognition of overlapping epitopes, whereas OKT3 and SK7 recognize distinct epitopes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested for flow cytometry using normal human peripheral blood cells
• Recommended Use: 5 µL (0.03 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
• Light Sensitivity: Protect from light (photo-oxidation sensitive)
• Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333)
• Excitation/Emission: 488 nm / 695 nm (Blue Laser)
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

The CD3 subunit complex is crucial for transducing antigen-recognition signals into T cells and regulating TCR surface expression. T cell activation through the TCR involves CD3γ, CD3δ, CD3ε, and CD3ζ subunits, which are structurally related members of the immunoglobulin superfamily encoded on chromosome 11. CD3 subunits associate with cytoplasmic signaling molecules via double tyrosine-based motifs. CD3 is expressed on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells. Reduced T lymphocyte percentages may occur in certain autoimmune diseases. Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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