Thermo Fisher Scientific Villin Recombinant Rabbit Monoclonal Antibody (VIL1, 4107R)
Recombinant rabbit monoclonal antibody specific for human Villin (VIL1, 4107R). Optimized for IHC (paraffin). Provides high specificity for gastrointestinal and pancreatic adenocarcinoma detection. Supplied as liquid, 200 µg/mL, stored at 4°C. For rese...
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Applications and Tested Dilution
- Immunohistochemistry (Paraffin) (IHC-P): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | VIL1, 4107R |
| Immunogen | Recombinant fragment (amino acids 600–700) of human Villin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For IHC (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues: Heat sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
Target Information
Villin is a cytoskeletal protein associated with the microvillar actin core bundle of gastrointestinal and renal brush borders. It interacts with actin in a Ca²⁺ and phosphoinositide-regulated manner. Villin serves as a highly specific marker for adenocarcinomas of the gastrointestinal tract and pancreas, and is also expressed in some Merkel cell tumors and adenocarcinomas of the lung, prostate, ovary, and kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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