Thermo Fisher Scientific Villin Recombinant Rabbit Monoclonal Antibody (VIL1, 4107R)
Recombinant rabbit monoclonal antibody for Villin (VIL1, 4107R). Validated for IHC (Paraffin) applications. Specific to human Villin protein. High purity (Protein A/G) and stable in PBS buffer. Ideal for gastrointestinal adenocarcinoma research.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | VIL1, 4107R |
| Immunogen | Recombinant fragment (around aa600–700) of human Villin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For IHC (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Villin is a cytoskeletal protein associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It interacts with actin in a Ca²⁺ and phosphoinositide-regulated manner. Villin serves as a specific marker for adenocarcinomas of the gastrointestinal tract and pancreas, and is also expressed in certain Merkel cell tumors and adenocarcinomas of lung, prostate, ovary, and kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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