
Thermo Fisher Scientific Villin Recombinant Rabbit Monoclonal Antibody (VIL1, 4107R)
사람 Villin 단백질을 인식하는 토끼 단클론 항체로, 면역조직화학(IHC) 분석에 적합합니다. HEK293 세포에서 발현된 재조합 항체이며, Protein A/G로 정제되었습니다. 위장관 및 췌장 선암 등에서 Villin 발현 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | VIL1, 4107R |
| Immunogen | Recombinant fragment (around aa600–700) of human Villin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed):
Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Villin is a cytoskeletal protein associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It interacts with actin in a Ca²⁺ and phosphoinositide-regulated manner. Villin is a specific marker for adenocarcinomas of the gastrointestinal tract and pancreas, and is also expressed in some Merkel cell tumors and adenocarcinomas of the lung, prostate, ovary, and kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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