Thermo Fisher Scientific PLK1 Monoclonal Antibody (OTI9E9), TrueMAB
인간 PLK1 단백질을 인식하는 Mouse IgG2b 단일클론 항체로, 세포 분열 및 암 발생 연구에 활용 가능. Western blot과 유세포분석에 적합하며, 고순도 친화 크로마토그래피 정제 제품. -20°C 보관, PBS/BSA/glycerol 완충액 포함.
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Applications
- Western Blot (WB): 1:500
- Flow Cytometry (Flow): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9E9 |
| Immunogen | Full length human recombinant protein of human PLEK produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.03 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
PLK1 is a serine/threonine kinase involved in cell mitosis and is regarded as a marker of cell proliferation. It plays a critical role in carcinogenesis. Polo-like kinase proteins act by binding and phosphorylating proteins that are already phosphorylated on a specific motif recognized by the POLO box domains. PLK1 phosphorylates multiple substrates including BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. It plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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